Survival and State of Garlic Explants of Two Lithuanian Cultivars after Cryopreservation

Cryopreservation features of Lithuanian garlic (Allium sativum L.) cultivars ‘Žiemiai’ and ‘Dangiai’ were investigated. The survival rate state explants, depending on the type explant, dehydration conditions evaluated in experiment. Explants isolated from cloves bulbils dehydrated with a plant vitrification solution 3 (PVS 3) containing 50% w/v glycerol sucrose liquid MS medium. Three different exposure durations 1.5, 2, h PVS3 applied at 0 °C. Unloaded stem domes planted Petri dishes medium supplemented 1 mg L−1 naphthaleneacetic acid (NAA) 6–dimethylallylamino purine (2-iP) 0.8% agar. obtained results showed that cryopreservation nitrogen reduced explants by 20–40%. average number surviving after freezing reached 64.3% for both cultivars. Cultivar 24% higher capability than ‘Dangiai’. total 76.1%. 23% more effective compared to cloves. Evaluation effect duration was highest treatment 91%. Treatment 2 sufficient Obtained confirm efficiency depends complex factors.